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There was Nos1 (decreased expression), Nos2 (increased expression), Plcg1 (decreased expression), Prkcg (increased, and then decreased expression), Ptgs2 (increased expression), and no effect on Nos3 and Ptk2

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Wash your hands thoroughly and sanitize the injection site with an alcohol pad, letting it air-dry completely—this step improves needle glide and reduces tissue trauma