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The homogenate was centrifuged at 12,000 rpm for 15 min at 4°C and the supernatant was collected for assay MPO activity as determined by measuring the H 2 O 2 -dependent oxidation of o -dianisidine solution (3,3′-dimethoxybenzidine dihydrochloride in potassium phosphate buffer, pH 6.0) at 450 nm using an Epoch microplate spectrophotometer (BioTek, VT, USA)